ΠΡΟΚΛΗΣΗ ΚΥΤΤΑΡΟΓΕΝΝΗΤΙΚΩΝ ΚΑΙ ΑΝΤΙΝΕΟΠΛΑΣΜΑΤΙΚΩΝ ΒΛΑΒΩΝ ΣΕ ΑΝΘΡΩΠΙΝΑ ΛΕΜΦΟΚΥΠΑΡΑ ΙΝ VITRO ΚΑΙ ΣΕ ΑΣΚΙΤΙΚΑ ΚΑΡΚΙΝΙΚΑ ΚΥΤΤΑΡΑ ΤΟΥ EHRLICH ΙΝ VIVO
Keywords:
CAF, caffeine, CPZ, chlorpormazine, Ehrlich ascites tumor, EAT, milotic index, ΜΙ, mitomycin C, MMC, cell cycle delays or proliferating rate index, PRI, sister chromatid exchanges, SCEs,Abstract
Chlorpromazine (CPZ), a potent phenothiazine tranquilizer, is now emerging as an adjuvant chemotherapeutic agent for the treatment of neoplasia. There are conflicting reports for positive and negative results of CPZ action in human cancer cells.
These results stimulated our study, which was set up to investigate: a) the potential of CPZ, alone or in combination with Mitomycin C (MMC) and/or Caffeine (CAF), to induce cytogenetic effects οn human lymphocytes in vitro; b) the ability of CPZ, alone or in combination with MMC, to cause cytogenetic damage οn Ehrlich Ascites tumor (ΕΑΤ) cells in νίνο; and c) an antitumour study of the survival time and the ascitic volume in ΕΑΤ bearing Balb/C mice.
Sister Chromatid Exchanges (SCEs) have been proposed as a very sensitive method for detecting mutagens and/or carcinogens and further more as a method of evaluating chemotherapy in vitro and in vivo. Also, studies have shown that the determination of proliferation rates and milotic indices should be useful and very sensitive indicators of the cellular toxicity.
The result indicated that: (a) the combination of CPZ plus CAF and MMC exerted strong cytostatic and cytotoxic action in cultured human lymphocytes, (b) the combination of CPZ plus MMC exerted strong cytostatic and cytotoxic action in ΕΑΤ cells in vivo, and (c) the double combination CPZ plus MMC significantly increased the survival span of the mice inoculated with ΕΑΤ cells and reduced the increase of the weight of the tumors.
It is suggested that the combination of CPZ with various antineoplastic agents (like MMC) could achieve increased effectiveness, better therapy results and could be successfully applied in the treatment of various types of human cancer.
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